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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide ordinarily is made up of the epitope recognized because of the antibody.
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This information outlines additional feasible optimization of cellular and immunoassay parameters to make certain the very best results are...
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to here the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining from your blocked antibody versus the antibody by itself, one can see which staining is particular.
Concentrate on Details Cyclin E belongs for the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is also degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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